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vector pspcas9 bb 2a puro  (Addgene inc)


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    Structured Review

    Addgene inc vector pspcas9 bb 2a puro
    Vector Pspcas9 Bb 2a Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2902 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px459+vector/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/bio_rxiv__64898__2026__03__27__714679-182-17-19
    Average 96 stars, based on 2902 article reviews
    vector pspcas9 bb 2a puro - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Nutrient Availability Dictates Cancer Metabolism-Based Therapeutic Responses to Non-oncology Drugs
    Article Snippet: .. The custom-designed gRNA sequences targeting GPI, AMPKα1, 123 AMPKα2, LATS1, LATS2, and LKB1 were cloned into the px459 vector (Addgene plasmid No. 62988). ..

    Article Title: Multi-omics dissection of the genetic regulation underlying fatty acid composition in cattle
    Article Snippet: .. These sgRNAs were then cloned into the px459 vector (Addgene, 48139). ..

    Article Title: NSD1-Mediated PPARγ Methylation Enhances PTEN Activity to Suppress Glycolysis and Tumor Progression in Endometrial Cancer
    Article Snippet: .. 129 Single-guide RNAs targeting coding regions of the respective genes were designed and cloned 130 into the PX459 vector (Addgene plasmid #48139; RRID: Addgene_48139). ..

    Article Title: Deubiquitinase OTULIN dampens RIG-I-dependent antiviral signaling by removing linear ubiquitination from TRAF6.
    Article Snippet: .. Human OTULIN gRNA was cloned into the PX459 vector (Addgene, #62988). .. Mice Otulin gRNA was cloned into the lentiCRISPR v2 vector (Addgene, #52961).

    Article Title: Multi-omics dissection of the genetic regulation underlying fatty acid composition in cattle.
    Article Snippet: .. These sgRNAs were then cloned into the px459 vector (Addgene, 48139). ..

    Plasmid Preparation:

    Article Title: Nutrient Availability Dictates Cancer Metabolism-Based Therapeutic Responses to Non-oncology Drugs
    Article Snippet: .. The custom-designed gRNA sequences targeting GPI, AMPKα1, 123 AMPKα2, LATS1, LATS2, and LKB1 were cloned into the px459 vector (Addgene plasmid No. 62988). ..

    Article Title: Isoquercitrin Improves Renal Tubular Epithelial Cell Mitochondrial Dysfunction by Regulating Extracellular Signal-Regulated Kinase/Reactive Oxygen Species Signaling and Connexin 43 Expression in Diabetic Kidney Disease.
    Article Snippet: Isoquercitrin (IQ) is a natural flavonoid compound with potent antiinflammatory and antioxidant effects; its role and mechanism of action in diabetic kidney disease (DKD) are unclear.. This study clarified the role of IQ in delaying DKD and regulating tubular epithelial cell lipid metabolism. db/db mice were randomly divided into DKD, lowdose IQ, highdose IQ, and dapagliflozin groups.. C57BL/6J mice were used as controls.

    Article Title: Mitorubin, berberrubine-based compounds that improve mitochondrial function, exhibit cardioprotective effects against age-related cardiac dysfunction.
    Article Snippet: .. The oligonucleotide pair corresponding to the mMITOL guide RNA (gRNA sequence, 5′-TCAACAGATGCTGGACAGGT-3′) was annealed and ligated into the PX459 vector (48139, Addgene). .. C2C12 cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific), and knockout clones were selected by serial dilution.

    Article Title: Multi-omics dissection of the genetic regulation underlying fatty acid composition in cattle
    Article Snippet: .. These sgRNAs were then cloned into the px459 vector (Addgene, 48139). ..

    Article Title: NSD1-Mediated PPARγ Methylation Enhances PTEN Activity to Suppress Glycolysis and Tumor Progression in Endometrial Cancer
    Article Snippet: .. 129 Single-guide RNAs targeting coding regions of the respective genes were designed and cloned 130 into the PX459 vector (Addgene plasmid #48139; RRID: Addgene_48139). ..

    Article Title: Deubiquitinase OTULIN dampens RIG-I-dependent antiviral signaling by removing linear ubiquitination from TRAF6.
    Article Snippet: .. Human OTULIN gRNA was cloned into the PX459 vector (Addgene, #62988). .. Mice Otulin gRNA was cloned into the lentiCRISPR v2 vector (Addgene, #52961).

    Article Title: Proximal proteomics analysis reveals DNA polymerase δ subunit 3 is a new MCM2 binding partner and promotes parental histones inheritance in mammalian cells.
    Article Snippet: .. Oligonucleotides were synthesized and inserted into pX459 vector (Addgene cat. no. 48139). ..

    Article Title: Multi-omics dissection of the genetic regulation underlying fatty acid composition in cattle.
    Article Snippet: .. These sgRNAs were then cloned into the px459 vector (Addgene, 48139). ..

    Purification:

    Article Title: Isoquercitrin Improves Renal Tubular Epithelial Cell Mitochondrial Dysfunction by Regulating Extracellular Signal-Regulated Kinase/Reactive Oxygen Species Signaling and Connexin 43 Expression in Diabetic Kidney Disease.
    Article Snippet: Isoquercitrin (IQ) is a natural flavonoid compound with potent antiinflammatory and antioxidant effects; its role and mechanism of action in diabetic kidney disease (DKD) are unclear.. This study clarified the role of IQ in delaying DKD and regulating tubular epithelial cell lipid metabolism. db/db mice were randomly divided into DKD, lowdose IQ, highdose IQ, and dapagliflozin groups.. C57BL/6J mice were used as controls.

    Transformation Assay:

    Article Title: Isoquercitrin Improves Renal Tubular Epithelial Cell Mitochondrial Dysfunction by Regulating Extracellular Signal-Regulated Kinase/Reactive Oxygen Species Signaling and Connexin 43 Expression in Diabetic Kidney Disease.
    Article Snippet: Isoquercitrin (IQ) is a natural flavonoid compound with potent antiinflammatory and antioxidant effects; its role and mechanism of action in diabetic kidney disease (DKD) are unclear.. This study clarified the role of IQ in delaying DKD and regulating tubular epithelial cell lipid metabolism. db/db mice were randomly divided into DKD, lowdose IQ, highdose IQ, and dapagliflozin groups.. C57BL/6J mice were used as controls.

    Polymerase Chain Reaction:

    Article Title: Isoquercitrin Improves Renal Tubular Epithelial Cell Mitochondrial Dysfunction by Regulating Extracellular Signal-Regulated Kinase/Reactive Oxygen Species Signaling and Connexin 43 Expression in Diabetic Kidney Disease.
    Article Snippet: Isoquercitrin (IQ) is a natural flavonoid compound with potent antiinflammatory and antioxidant effects; its role and mechanism of action in diabetic kidney disease (DKD) are unclear.. This study clarified the role of IQ in delaying DKD and regulating tubular epithelial cell lipid metabolism. db/db mice were randomly divided into DKD, lowdose IQ, highdose IQ, and dapagliflozin groups.. C57BL/6J mice were used as controls.

    Sequencing:

    Article Title: Mitorubin, berberrubine-based compounds that improve mitochondrial function, exhibit cardioprotective effects against age-related cardiac dysfunction.
    Article Snippet: .. The oligonucleotide pair corresponding to the mMITOL guide RNA (gRNA sequence, 5′-TCAACAGATGCTGGACAGGT-3′) was annealed and ligated into the PX459 vector (48139, Addgene). .. C2C12 cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific), and knockout clones were selected by serial dilution.

    Synthesized:

    Article Title: Proximal proteomics analysis reveals DNA polymerase δ subunit 3 is a new MCM2 binding partner and promotes parental histones inheritance in mammalian cells.
    Article Snippet: .. Oligonucleotides were synthesized and inserted into pX459 vector (Addgene cat. no. 48139). ..



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